Indigofera zollingeriana as an Alternative Feed and Immunostimulant For Snakehead Fish Infected with Aeromonas hydrophila
DOI:
https://doi.org/10.30736/grouper.v17i1.370Keywords:
Aeromonas hydrophila, Channa striata, Fish feed, immunostimulant, Indigofera zollingerinaAbstract
The demand for snakehead fish in South Sumatra continues to increase, encouraging farmers to develop intensive cultivation. However, the main challenges of high feed costs and disease attacks need to be addressed to maintain optimal productivity. One alternative being studied is the use of Indigofera zollingeriana as a source of protein and immunostimulant in feed. This study aims to evaluate the effect of substituting fish meal with Indigofera flour on the immune response of snakehead fish (Channa striata) infected with Aeromonas hydrophila bacteria. The study used a Completely Randomized Design with four treatments of fish meal substitution with Indigofera zollingeriana flour, namely 0%, 15%, 30%, and 45%. The parameters observed included phytochemical tests, growth, blood profile, and water quality. The results of the phytochemical test showed the presence of flavonoids, alkaloids, tannins, and saponins. In the blood profile, the 45% treatment produced the highest erythrocyte value (4.54 × 10⁴ cells) and hematocrit of 38%. The best growth was shown in the 30% treatment with an SGR value of 2.56±0.23d and an FCR of 1.61±0.11a, while the highest survival rate (84.44%) was obtained in the 45% treatment. These findings indicate that Indigofera has the potential as a functional feed to improve the immunity and productivity of snakehead fish.
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References
Abdullah L. (2014). Prospektif Agronomi dan Ekofisiologi Indigofera Zollingerina Sebagai Tanaman Penghasil Hijauan Pakan Berkualitas Tinggi. Pastura: Journal of Tropical Forage Science. 3(2): 79 – 83.
Alagbe JO. (2020). Chemical Evaluation Of Proximate, Vitamin and Amino Acid Profile of Leaf, Stem Bark and Root Of Indigofera Tinctoria. European Journal Research Development and Sustainability (EJRDS).; 1(1): 6 – 12.
Geromettaa, Elise., I. Grondina., J. Smadjaa., M. Frederich, A. Gauvin-Bialeckia. (2020). A review Of Traditional Uses, Phytochemistry and Pharmacology of The Genus Indigofera. Elsevier. 1(1):1-82
Hastuti, A. W., D. Samsudewa, E. T. Setiatin. (2020). Pengaruh Penambahan Indigofera zollingeriana dalam Stock Solutionterhadap Motilitas dan Abnormalitas Spermatozoa Kambing Peranakan Etawa (PE). Jurnal Sain Peternakan Indonesia. 15(2):167-172
Herawati, V. E., Nanda,I., Pinandoyo, Hutabarat, J., Radjasa, O. K., Wirasatriya,A., Nugroho. (2021). Striped snakehead fish (Channa striata) feed utilizing maggot meal substitution for fish meal: Nutrient content, effects on growth, and feed utilization efficiency. Songklanakarin J. Sci. Technol. 43 (3): 855-863
Huaa, K., Koppeb, W. dan Fontanillas., (2019). R. Effects of dietary protein and lipid levels on growth, body composition and nutrient utilization of Channa striata. Aquaculture 5(1): 368–373
Hutapea, P. S., L. Abdullah., P. D. M. H. Karti, I. Anas. (2018). Improvement of Indigofera zollingeriana Production and Methionine Content through Inoculation of Nitrogen-Fixing Bacteria. 41(1):37-45
Javeda, F., Q. Jabeena., N. Aslam dan A. M. Awan. (2020). Pharmacological evaluation of analgesic, anti-inflammatory and antipyretic activities of ethanolic extract of Indigofera argentea Burm. Journal of Ethnopharmacology. 259 (1):1-8. https://doi.org/10.1016/j.jep.2020.112966
Jefry., Mia Setiawati, Dedi Jusadi dan Ichsan Achmad Fauzi. (2021). Cellulase Hydrolyzed Indigofera Zolingeriana Leaf Utilization as A Feed Ingredient For Gourami Fingerling. Jurnal Akuakultur Indonesia. 20 (2): 139–147.
Karakousi, Christina-Vasiliki., Chrysi Gabrieli, Eugene Kokkalou. (2018). Chemical composition and biological activities of Indigofera hirsuta aerial parts’ methanol fractions. Natural Product Research. 32(1):1-6. https://doi.org/10.1080/14786419.2018.1489390
Kementerian Kelautan dan Perikanan. (2020). Langkah KKP Kembangkan Industri Budidaya Ikan Gabus Sebagai Komoditas Unggulan Berbasis Lokal. [Internet]. [cited 2023 April 06] Available from: https://kkp.go.id/artikel/23431-langkah-kkp-kembangkan-industri-budidaya-ikan-gabus-sebagai-komoditas-unggulan-berbasis-lokal.
Kong, Yidi., Li, Min, Xiaofeng Shan, Guiqin Wang, Guanghong Han. (2021). Effects of deltamethrin subacute exposure in snakehead fish, Channa argus: Biochemicals, antioxidants and immune responses. Ecotoxicology and Environmental Safety. 209(1):1-9.
Li Lili, Ru Yao, Rikke Heidemann Olsen, Yan Zhang, Hecheng Meng.(2022). Antibiotic Resistance And Polymyxin B Resistance Mechanism Of Aeromonas Spp. Isolated From Yellow Catfish, Hybrid Snakeheads And Associated Water From Intensive Fish Farms In Southern China. LWT- Food Science and Technology.; 166(1):1-9
Mukti, R.C., Yonarta, D., Pangawikan, A.D. (2019). Pemanfaatan Daun Indigofera Zollingerina Sebagai Bahan Pakan Ikan Patin Pangasius Sp. J.Ilmu-Ilmu Perairan, Pesisir dan Perikanan 8(1): 18-25. doi: 10.13170/depik.8.1.13056.
Muslim M, Iskandar A, Hendriana A, Lutfi L. (2021).Performa pertumbuhan calon induk ikan gabus (Channa striata) yang diberi pakan benih ikan nila (Oreochromis sp) J.Sains Terapan. 11(1): 1-8.
Mutmainnah D. (2013).Hubungan panjang berat dan faktor kondisi ikan gabus (Channa striata Bloch,1793) yang dibesarkan di rawa lebak provinsi Sumatera Selatan. Depik. 2013; 2(3) : 184 – 190.
Nursatia, Sarjito, A. H. C. Haditomo. (2017). Pemberian Ekstrak Bawang Putih Dalam Pakan Sebagai Imunostimulan Terhadap Kelulushidupan Dan Profil Darah Ikan Patin (Pangasius sp.). Journal of Aquaculture Management and Technology. 6(3): 234-241
Palupi R, Abdullah L, Astuti DA, Sumiati. (2014). Potensi dan pemanfaatan tepung pucuk Indigofera sp. sebagai bahan pakan substitusi bungkil kedelai dalam ransum ayam petelur. JITV 19(3): 210-219. DOI: http://dx.doi.org/10.14334/jitv.v19i3.1084
Palupi, Rizki, F. N. L. Lubis, A. N. T. Pratama. (2022). The effect of the use of cassava tuber (Manihot esculenta) and Indigofera zollingeriana leaf flour combination as a source of energy supplemented with citric acid in ration on broiler small intestine characteristics and productivity. 9(3): 471–480
Pratama, E. R., B. Putri, L. Abdullah., Indra Gumay Yudha dan Dwi Mulyasih. (2019). Penambahan Tepung Pucuk Indigofera zollingeriana (Miquel, 1855) Dalam Pakan Untuk Meningkatkan Kualitas Warna Ikan Sumatra Puntigrus tetrazone (Bleeker, 1855). Jurnal Rekayasa dan Teknologi Budidaya Peraira. 7(2):889-896
Ramadhan, M. K, Arimbi, Sarmanu. (2016) Efek Perendaman Ekstrak Spirulina platensis sebagai Imunostimulan terhadap Gambaran Histopatologi Usus Ikan Gurame (Osphronemus gouramy) yang diinfeksi Aeromonas hydrophila. Veterina Medika. 9(3):1-6
Santi, M. A. (2017). Penggunaan Tepung Pucuk Indigofera Zollingeriana Sebagai Pengganti Bungkil Kedelai Dalam Ransum Dan Pengaruhnya Terhadap Kesehatan Ayam Broiler. Jurnal Peternakan. 1(2):17-22.
Santi, M. A. (2018). Pengaruh Substitusi Protein Tepung Pucuk Indogofera Zollingeriana Dengan Protein Bungkil Kedelai Dalam Ransum Terhadap Organ Dalam Broiler. Jurnal Peternakan. 2(2):15-20
Shulikin,A.N., Syahrizal, dan Safratilofa. (2021). Pengaruh tepung daun Indigofera (Indigofera zollingerina) sebagai substitusi bahan baku pakan mandiri terhadap laju pertumbuhan benih ikan gurame (Osphronemus gouramy). J.Akuakultur Sungai dan Danau, 6(2): 68-73.
Suharlina, Abdullah, L., Lubis, A.D. (2019). Kualitas nutrisi hijauan (Indigofera zollingerina) yang diberi pupuk organic cair asal limbah industry penyedap masakan. J.Pertanian Terpadu, 7(1): 28-37. Retrieved from http://ojs.stiperkutim.ac.id/index.php/jpt/article/view/179
Talha. Muhammad., Islam. Noor Ul., Z. Muhammad., S. Abdul., N. Asif., K. Farhat Ali., (2022). Biological Evaluation, Phytochemical Screening, and Fabrication Of Indigofera Linifolia Leaves Extract-Loaded Nanoparticles. Molecules. 27(1):1-20. https://doi.org/10.3390/molecules27154707
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