In Vitro Antioxidant Activity Dpph Assay of Protein Hydrolysate Mackerel (Scomber Scombrus) Viscera

Windra Hutama Putra; Sri Subekti; Muhammad Amin

doi:10.30736/grouper.v15i2.266

Authors

Windra Hutama Putra Fisheries Science Master Program, Faculty of Fisheries and Marine, Airlangga University
Sri Subekti Marine Department, Faculty of Fisheries and Marine and Entomology Study Group, Institute Tropical Disease, Airlangga University
Muhammad Amin Aquaculture Department, Faculty of Fisheries and Marine, Airlangga University

DOI:

https://doi.org/10.30736/grouper.v15i2.266

Keywords:

Mackerel fish, Alcalase enzyme, Molecular weight, Antioxidant activity

Abstract

Based on exports data mackerel (Scomber sombrus) in 2023 approximately 2,702 tons, while by-products had produced around 60%. This creates serious problems with its disposal. Fish by-products can be processed into value-added products, one of which is fish protein hydrolysates. The purpose of the study was to determine the molecular weight of the SDS-PAGE (Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis) method and the antioxidant activity DPPH assay (2,2-diphenyl-1-picrylhydrazil) hydrolysate of mackerel viscera protein. The results showed a molecular weight in the range of 12 – 16 kDa. Antioxidant activity was shown by an inhibition percentage value of 50.117 ± 0.4793 % with an antioxidant capacity of 0.9141 ± 0.0606 mmol TE/g of Trolox equivalent samples. Based on antioxidant activity, mackerel viscera protein hydrolysate has the potential to be a natural source of antioxidants.

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References

Abdelhedi, O., Jridi, M., Jemil, I., Mora, L., Toldrá, F., Aristoy, M. C., Boualga, A., Nasri, M., & Nasri, R. (2016). Combined biocatalytic conversion of smooth hound viscera: Protein hydrolysates elaboration and assessment of their antioxidant, anti-ACE and antibacterial activities. Food Research International, 86, 9-23.

Agustin, Venny., Putra, M. M., & Husni, A. (2023). Impact of enzymatic hydrolysis on antioxidant activity of snakehead fish (Channa striata) head protein hydrolysate. Jurnal Ilmiah Perikanan dan Kelautan, 15(1): 44-56.